1x tbs ihc wash buffer with tween 20 cell marque cat Search Results


1x t4 ligase buffer  (New England Biolabs)
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New England Biolabs 1x t4 ligase buffer
1x T4 Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x digest buffer  (Zymo Research)
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Zymo Research 1x digest buffer
1x Digest Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x biorad 9 hercules ca cat 1610743  (Bio-Rad)
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Bio-Rad 1x biorad 9 hercules ca cat 1610743
1x Biorad 9 Hercules Ca Cat 1610743, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs  (HY Labs)
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HY Labs pbs
Pbs, supplied by HY Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snail1  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc snail1
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
Snail1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris buffered saline biorad  (Bio-Rad)
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Bio-Rad tris buffered saline biorad
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
Tris Buffered Saline Biorad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ripa lysis buffer  (Thermo Fisher)
99
Thermo Fisher ripa lysis buffer
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
Ripa Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x mem non essential amino acids solution  (Thermo Fisher)
86
Thermo Fisher 1x mem non essential amino acids solution
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
1x Mem Non Essential Amino Acids Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x ripa cell lysis solution  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc 1x ripa cell lysis solution
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
1x Ripa Cell Lysis Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x sodium citrate buffer  (Millipore)
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Millipore 1x sodium citrate buffer
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
1x Sodium Citrate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x ack lysis buffer  (Thermo Fisher)
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Thermo Fisher 1x ack lysis buffer
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
1x Ack Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x citrate buffer  (Agilent technologies)
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Agilent technologies 1x citrate buffer
Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for <t>Snail1</t> in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).
1x Citrate Buffer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for Snail1 in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).

Journal: Cell death & disease

Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma.

doi: 10.1038/s41419-024-06733-3

Figure Lengend Snippet: Fig. 5 AP2ε regulates phenotypic plasticity of melanoma cells. A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; *p < 0.05; ns: not significant (Two-way- ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for Snail1 in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε-/-/Tg(GRM1) and Tg(GRM1) cells (n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; *p < 0.05 (Two-tailed Student’s t-test; ns: not significant).

Article Snippet: Primary antibodies against MITF (1:500 in 5% BSA, Santa Cruz, sc-515925, RRID:AB_2828036), BRN2 (1:500 in 5% BSA/1x TBS-T, Santa Cruz, sc393324, RRID:AB_2737347), Snail1 (1:1,000 in 5% BSA/1x TBS-T, Cell Signaling Technology Cat# 3879, RRID:AB_2255011) and E-cadherin (1:1,000 in 5% BSA/1x TBS-T, Cell Signaling Technology Cat# 3195, RRID:AB_2291471) were incubated overnight at 4 °C.

Techniques: Western Blot, Transfection, Over Expression, Plasmid Preparation, Control, Immunohistochemical staining, Staining, Expressing, Two Tailed Test